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il 1r1  (Jackson Laboratory)
86
Jackson Laboratory il 1r1
The early life IL-17 response is dependent on IL-1 β produced by infiltrating myeloid cells. (A) Differential abundance using MiloR analysis of single cell sequencing of total CD45+ cells of weanlings and adults. (B, C) Total number of neutrophils(B) and monocytes (C) in the colon normalized to weight of the tissue of mice at indicated ages. (D, E) Total number of neutrophils (D) and monocytes (E) in the colon lamina propria of 3-week old littermate IL-17+/-and IL17-/-mice. (F) UMAP of Il1b expression in weanling and adult colon CD45+ cells. (G) Expression of Il1b in total colon of mice at indicated ages. (H, I) Representative flow plot (H) and percent (I) of pro-IL-1β+ myeloid cells in the LP of weanling and adult colons. (J) ELISA quantification of IL-1β in the supernatant of colon explants of weanling and adult mice, normalized to weight of tissue. (K, L) Percent of IL-17+ γδ T cells (K) and percent of neutrophils (L) in colonic LP of control mice and mice treated with anti-IL-1R in vivo antibody from 2-3 weeks. (M, N) Percent of IL-17+ γδ T cells (M) and percent of neutrophils (N) in colonic LP of littermate IL-1R+/-and IL-1R-/-at 3 weeks of age. (O) UMAP of type 3 immunity score and Il1r1 expression on total thymocytes from day 0 mouse (P) Percent of IL-17+ γδ T cells in 3-week-old TCRδ-/-adoptively transferred with IL-1R+/-or IL-1R-/-thymus cells. (Q) UMAP of <t>IL-1R1</t> expression in total CD45+ cells in human fetal thymus γδ T cells. (R, S ) Percent of IFNγ (R) and IL-17 (S) of Vγ9+ γδ T cells in human cord blood following stimulation with IL-1β and zoledronate. (B, C, D, E, G, I-N, P, R, and S) Each dot represents cells from an individual mouse. Data are represented as mean with SD. Graphs are pooled data from at least two independent experiments with atleast n = 3 in each experiment. (H) Contour plots are representative at least two independent experiments. Numbers indicate frequency of cells within each quadrant or box. (B, C, G) Data were analyzed using Kruskal-Wallis ANOVA test or (D, E, I-N and P) Mann-Whitney non-parametric unpaired T test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p <0.0001).
Il 1r1, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il 1r1  (ABclonal Biotechnology)
94
ABclonal Biotechnology il 1r1
The early life IL-17 response is dependent on IL-1 β produced by infiltrating myeloid cells. (A) Differential abundance using MiloR analysis of single cell sequencing of total CD45+ cells of weanlings and adults. (B, C) Total number of neutrophils(B) and monocytes (C) in the colon normalized to weight of the tissue of mice at indicated ages. (D, E) Total number of neutrophils (D) and monocytes (E) in the colon lamina propria of 3-week old littermate IL-17+/-and IL17-/-mice. (F) UMAP of Il1b expression in weanling and adult colon CD45+ cells. (G) Expression of Il1b in total colon of mice at indicated ages. (H, I) Representative flow plot (H) and percent (I) of pro-IL-1β+ myeloid cells in the LP of weanling and adult colons. (J) ELISA quantification of IL-1β in the supernatant of colon explants of weanling and adult mice, normalized to weight of tissue. (K, L) Percent of IL-17+ γδ T cells (K) and percent of neutrophils (L) in colonic LP of control mice and mice treated with anti-IL-1R in vivo antibody from 2-3 weeks. (M, N) Percent of IL-17+ γδ T cells (M) and percent of neutrophils (N) in colonic LP of littermate IL-1R+/-and IL-1R-/-at 3 weeks of age. (O) UMAP of type 3 immunity score and Il1r1 expression on total thymocytes from day 0 mouse (P) Percent of IL-17+ γδ T cells in 3-week-old TCRδ-/-adoptively transferred with IL-1R+/-or IL-1R-/-thymus cells. (Q) UMAP of <t>IL-1R1</t> expression in total CD45+ cells in human fetal thymus γδ T cells. (R, S ) Percent of IFNγ (R) and IL-17 (S) of Vγ9+ γδ T cells in human cord blood following stimulation with IL-1β and zoledronate. (B, C, D, E, G, I-N, P, R, and S) Each dot represents cells from an individual mouse. Data are represented as mean with SD. Graphs are pooled data from at least two independent experiments with atleast n = 3 in each experiment. (H) Contour plots are representative at least two independent experiments. Numbers indicate frequency of cells within each quadrant or box. (B, C, G) Data were analyzed using Kruskal-Wallis ANOVA test or (D, E, I-N and P) Mann-Whitney non-parametric unpaired T test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p <0.0001).
Il 1r1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+1r1/IL1R1+Rabbit+pAb/10__1016_slash_j__gendis__2026__102250-88-63-64
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rabbit anti il 1r1  (Proteintech)
96
Proteintech rabbit anti il 1r1
The early life IL-17 response is dependent on IL-1 β produced by infiltrating myeloid cells. (A) Differential abundance using MiloR analysis of single cell sequencing of total CD45+ cells of weanlings and adults. (B, C) Total number of neutrophils(B) and monocytes (C) in the colon normalized to weight of the tissue of mice at indicated ages. (D, E) Total number of neutrophils (D) and monocytes (E) in the colon lamina propria of 3-week old littermate IL-17+/-and IL17-/-mice. (F) UMAP of Il1b expression in weanling and adult colon CD45+ cells. (G) Expression of Il1b in total colon of mice at indicated ages. (H, I) Representative flow plot (H) and percent (I) of pro-IL-1β+ myeloid cells in the LP of weanling and adult colons. (J) ELISA quantification of IL-1β in the supernatant of colon explants of weanling and adult mice, normalized to weight of tissue. (K, L) Percent of IL-17+ γδ T cells (K) and percent of neutrophils (L) in colonic LP of control mice and mice treated with anti-IL-1R in vivo antibody from 2-3 weeks. (M, N) Percent of IL-17+ γδ T cells (M) and percent of neutrophils (N) in colonic LP of littermate IL-1R+/-and IL-1R-/-at 3 weeks of age. (O) UMAP of type 3 immunity score and Il1r1 expression on total thymocytes from day 0 mouse (P) Percent of IL-17+ γδ T cells in 3-week-old TCRδ-/-adoptively transferred with IL-1R+/-or IL-1R-/-thymus cells. (Q) UMAP of <t>IL-1R1</t> expression in total CD45+ cells in human fetal thymus γδ T cells. (R, S ) Percent of IFNγ (R) and IL-17 (S) of Vγ9+ γδ T cells in human cord blood following stimulation with IL-1β and zoledronate. (B, C, D, E, G, I-N, P, R, and S) Each dot represents cells from an individual mouse. Data are represented as mean with SD. Graphs are pooled data from at least two independent experiments with atleast n = 3 in each experiment. (H) Contour plots are representative at least two independent experiments. Numbers indicate frequency of cells within each quadrant or box. (B, C, G) Data were analyzed using Kruskal-Wallis ANOVA test or (D, E, I-N and P) Mann-Whitney non-parametric unpaired T test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p <0.0001).
Rabbit Anti Il 1r1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+1r1/PGC1a+Antibody/pm41905731-154-27-30
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hek blue il 1r1 reporter cell line  (InvivoGen)
95
InvivoGen hek blue il 1r1 reporter cell line
The early life IL-17 response is dependent on IL-1 β produced by infiltrating myeloid cells. (A) Differential abundance using MiloR analysis of single cell sequencing of total CD45+ cells of weanlings and adults. (B, C) Total number of neutrophils(B) and monocytes (C) in the colon normalized to weight of the tissue of mice at indicated ages. (D, E) Total number of neutrophils (D) and monocytes (E) in the colon lamina propria of 3-week old littermate IL-17+/-and IL17-/-mice. (F) UMAP of Il1b expression in weanling and adult colon CD45+ cells. (G) Expression of Il1b in total colon of mice at indicated ages. (H, I) Representative flow plot (H) and percent (I) of pro-IL-1β+ myeloid cells in the LP of weanling and adult colons. (J) ELISA quantification of IL-1β in the supernatant of colon explants of weanling and adult mice, normalized to weight of tissue. (K, L) Percent of IL-17+ γδ T cells (K) and percent of neutrophils (L) in colonic LP of control mice and mice treated with anti-IL-1R in vivo antibody from 2-3 weeks. (M, N) Percent of IL-17+ γδ T cells (M) and percent of neutrophils (N) in colonic LP of littermate IL-1R+/-and IL-1R-/-at 3 weeks of age. (O) UMAP of type 3 immunity score and Il1r1 expression on total thymocytes from day 0 mouse (P) Percent of IL-17+ γδ T cells in 3-week-old TCRδ-/-adoptively transferred with IL-1R+/-or IL-1R-/-thymus cells. (Q) UMAP of <t>IL-1R1</t> expression in total CD45+ cells in human fetal thymus γδ T cells. (R, S ) Percent of IFNγ (R) and IL-17 (S) of Vγ9+ γδ T cells in human cord blood following stimulation with IL-1β and zoledronate. (B, C, D, E, G, I-N, P, R, and S) Each dot represents cells from an individual mouse. Data are represented as mean with SD. Graphs are pooled data from at least two independent experiments with atleast n = 3 in each experiment. (H) Contour plots are representative at least two independent experiments. Numbers indicate frequency of cells within each quadrant or box. (B, C, G) Data were analyzed using Kruskal-Wallis ANOVA test or (D, E, I-N and P) Mann-Whitney non-parametric unpaired T test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p <0.0001).
Hek Blue Il 1r1 Reporter Cell Line, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+1r1/HEK-Blue+IL-1R+Cells/pmc13016199-106-10-14
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mil 1r1 fc  (R&D Systems)
94
R&D Systems mil 1r1 fc
The early life IL-17 response is dependent on IL-1 β produced by infiltrating myeloid cells. (A) Differential abundance using MiloR analysis of single cell sequencing of total CD45+ cells of weanlings and adults. (B, C) Total number of neutrophils(B) and monocytes (C) in the colon normalized to weight of the tissue of mice at indicated ages. (D, E) Total number of neutrophils (D) and monocytes (E) in the colon lamina propria of 3-week old littermate IL-17+/-and IL17-/-mice. (F) UMAP of Il1b expression in weanling and adult colon CD45+ cells. (G) Expression of Il1b in total colon of mice at indicated ages. (H, I) Representative flow plot (H) and percent (I) of pro-IL-1β+ myeloid cells in the LP of weanling and adult colons. (J) ELISA quantification of IL-1β in the supernatant of colon explants of weanling and adult mice, normalized to weight of tissue. (K, L) Percent of IL-17+ γδ T cells (K) and percent of neutrophils (L) in colonic LP of control mice and mice treated with anti-IL-1R in vivo antibody from 2-3 weeks. (M, N) Percent of IL-17+ γδ T cells (M) and percent of neutrophils (N) in colonic LP of littermate IL-1R+/-and IL-1R-/-at 3 weeks of age. (O) UMAP of type 3 immunity score and Il1r1 expression on total thymocytes from day 0 mouse (P) Percent of IL-17+ γδ T cells in 3-week-old TCRδ-/-adoptively transferred with IL-1R+/-or IL-1R-/-thymus cells. (Q) UMAP of <t>IL-1R1</t> expression in total CD45+ cells in human fetal thymus γδ T cells. (R, S ) Percent of IFNγ (R) and IL-17 (S) of Vγ9+ γδ T cells in human cord blood following stimulation with IL-1β and zoledronate. (B, C, D, E, G, I-N, P, R, and S) Each dot represents cells from an individual mouse. Data are represented as mean with SD. Graphs are pooled data from at least two independent experiments with atleast n = 3 in each experiment. (H) Contour plots are representative at least two independent experiments. Numbers indicate frequency of cells within each quadrant or box. (B, C, G) Data were analyzed using Kruskal-Wallis ANOVA test or (D, E, I-N and P) Mann-Whitney non-parametric unpaired T test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p <0.0001).
Mil 1r1 Fc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+1r1/Recombinant+Mouse+IL-1+RI+Fc+Chimera+Protein%2C+CF/pm41723527-109-41-42
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human il-1 ri fluorescein-conjugated antibody  (Bio-Techne corporation)
93
Bio-Techne corporation human il-1 ri fluorescein-conjugated antibody
The early life IL-17 response is dependent on IL-1 β produced by infiltrating myeloid cells. (A) Differential abundance using MiloR analysis of single cell sequencing of total CD45+ cells of weanlings and adults. (B, C) Total number of neutrophils(B) and monocytes (C) in the colon normalized to weight of the tissue of mice at indicated ages. (D, E) Total number of neutrophils (D) and monocytes (E) in the colon lamina propria of 3-week old littermate IL-17+/-and IL17-/-mice. (F) UMAP of Il1b expression in weanling and adult colon CD45+ cells. (G) Expression of Il1b in total colon of mice at indicated ages. (H, I) Representative flow plot (H) and percent (I) of pro-IL-1β+ myeloid cells in the LP of weanling and adult colons. (J) ELISA quantification of IL-1β in the supernatant of colon explants of weanling and adult mice, normalized to weight of tissue. (K, L) Percent of IL-17+ γδ T cells (K) and percent of neutrophils (L) in colonic LP of control mice and mice treated with anti-IL-1R in vivo antibody from 2-3 weeks. (M, N) Percent of IL-17+ γδ T cells (M) and percent of neutrophils (N) in colonic LP of littermate IL-1R+/-and IL-1R-/-at 3 weeks of age. (O) UMAP of type 3 immunity score and Il1r1 expression on total thymocytes from day 0 mouse (P) Percent of IL-17+ γδ T cells in 3-week-old TCRδ-/-adoptively transferred with IL-1R+/-or IL-1R-/-thymus cells. (Q) UMAP of <t>IL-1R1</t> expression in total CD45+ cells in human fetal thymus γδ T cells. (R, S ) Percent of IFNγ (R) and IL-17 (S) of Vγ9+ γδ T cells in human cord blood following stimulation with IL-1β and zoledronate. (B, C, D, E, G, I-N, P, R, and S) Each dot represents cells from an individual mouse. Data are represented as mean with SD. Graphs are pooled data from at least two independent experiments with atleast n = 3 in each experiment. (H) Contour plots are representative at least two independent experiments. Numbers indicate frequency of cells within each quadrant or box. (B, C, G) Data were analyzed using Kruskal-Wallis ANOVA test or (D, E, I-N and P) Mann-Whitney non-parametric unpaired T test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p <0.0001).
Human Il 1 Ri Fluorescein Conjugated Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il 1r1  (Biorbyt)
94
Biorbyt il 1r1
The early life IL-17 response is dependent on IL-1 β produced by infiltrating myeloid cells. (A) Differential abundance using MiloR analysis of single cell sequencing of total CD45+ cells of weanlings and adults. (B, C) Total number of neutrophils(B) and monocytes (C) in the colon normalized to weight of the tissue of mice at indicated ages. (D, E) Total number of neutrophils (D) and monocytes (E) in the colon lamina propria of 3-week old littermate IL-17+/-and IL17-/-mice. (F) UMAP of Il1b expression in weanling and adult colon CD45+ cells. (G) Expression of Il1b in total colon of mice at indicated ages. (H, I) Representative flow plot (H) and percent (I) of pro-IL-1β+ myeloid cells in the LP of weanling and adult colons. (J) ELISA quantification of IL-1β in the supernatant of colon explants of weanling and adult mice, normalized to weight of tissue. (K, L) Percent of IL-17+ γδ T cells (K) and percent of neutrophils (L) in colonic LP of control mice and mice treated with anti-IL-1R in vivo antibody from 2-3 weeks. (M, N) Percent of IL-17+ γδ T cells (M) and percent of neutrophils (N) in colonic LP of littermate IL-1R+/-and IL-1R-/-at 3 weeks of age. (O) UMAP of type 3 immunity score and Il1r1 expression on total thymocytes from day 0 mouse (P) Percent of IL-17+ γδ T cells in 3-week-old TCRδ-/-adoptively transferred with IL-1R+/-or IL-1R-/-thymus cells. (Q) UMAP of <t>IL-1R1</t> expression in total CD45+ cells in human fetal thymus γδ T cells. (R, S ) Percent of IFNγ (R) and IL-17 (S) of Vγ9+ γδ T cells in human cord blood following stimulation with IL-1β and zoledronate. (B, C, D, E, G, I-N, P, R, and S) Each dot represents cells from an individual mouse. Data are represented as mean with SD. Graphs are pooled data from at least two independent experiments with atleast n = 3 in each experiment. (H) Contour plots are representative at least two independent experiments. Numbers indicate frequency of cells within each quadrant or box. (B, C, G) Data were analyzed using Kruskal-Wallis ANOVA test or (D, E, I-N and P) Mann-Whitney non-parametric unpaired T test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p <0.0001).
Il 1r1, supplied by Biorbyt, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+1r1/IL1+Beta+antibody/pmc12881657-173-27-28
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interleukin 1 receptor  (Proteintech)
94
Proteintech interleukin 1 receptor
The early life IL-17 response is dependent on IL-1 β produced by infiltrating myeloid cells. (A) Differential abundance using MiloR analysis of single cell sequencing of total CD45+ cells of weanlings and adults. (B, C) Total number of neutrophils(B) and monocytes (C) in the colon normalized to weight of the tissue of mice at indicated ages. (D, E) Total number of neutrophils (D) and monocytes (E) in the colon lamina propria of 3-week old littermate IL-17+/-and IL17-/-mice. (F) UMAP of Il1b expression in weanling and adult colon CD45+ cells. (G) Expression of Il1b in total colon of mice at indicated ages. (H, I) Representative flow plot (H) and percent (I) of pro-IL-1β+ myeloid cells in the LP of weanling and adult colons. (J) ELISA quantification of IL-1β in the supernatant of colon explants of weanling and adult mice, normalized to weight of tissue. (K, L) Percent of IL-17+ γδ T cells (K) and percent of neutrophils (L) in colonic LP of control mice and mice treated with anti-IL-1R in vivo antibody from 2-3 weeks. (M, N) Percent of IL-17+ γδ T cells (M) and percent of neutrophils (N) in colonic LP of littermate IL-1R+/-and IL-1R-/-at 3 weeks of age. (O) UMAP of type 3 immunity score and Il1r1 expression on total thymocytes from day 0 mouse (P) Percent of IL-17+ γδ T cells in 3-week-old TCRδ-/-adoptively transferred with IL-1R+/-or IL-1R-/-thymus cells. (Q) UMAP of <t>IL-1R1</t> expression in total CD45+ cells in human fetal thymus γδ T cells. (R, S ) Percent of IFNγ (R) and IL-17 (S) of Vγ9+ γδ T cells in human cord blood following stimulation with IL-1β and zoledronate. (B, C, D, E, G, I-N, P, R, and S) Each dot represents cells from an individual mouse. Data are represented as mean with SD. Graphs are pooled data from at least two independent experiments with atleast n = 3 in each experiment. (H) Contour plots are representative at least two independent experiments. Numbers indicate frequency of cells within each quadrant or box. (B, C, G) Data were analyzed using Kruskal-Wallis ANOVA test or (D, E, I-N and P) Mann-Whitney non-parametric unpaired T test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p <0.0001).
Interleukin 1 Receptor, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The early life IL-17 response is dependent on IL-1 β produced by infiltrating myeloid cells. (A) Differential abundance using MiloR analysis of single cell sequencing of total CD45+ cells of weanlings and adults. (B, C) Total number of neutrophils(B) and monocytes (C) in the colon normalized to weight of the tissue of mice at indicated ages. (D, E) Total number of neutrophils (D) and monocytes (E) in the colon lamina propria of 3-week old littermate IL-17+/-and IL17-/-mice. (F) UMAP of Il1b expression in weanling and adult colon CD45+ cells. (G) Expression of Il1b in total colon of mice at indicated ages. (H, I) Representative flow plot (H) and percent (I) of pro-IL-1β+ myeloid cells in the LP of weanling and adult colons. (J) ELISA quantification of IL-1β in the supernatant of colon explants of weanling and adult mice, normalized to weight of tissue. (K, L) Percent of IL-17+ γδ T cells (K) and percent of neutrophils (L) in colonic LP of control mice and mice treated with anti-IL-1R in vivo antibody from 2-3 weeks. (M, N) Percent of IL-17+ γδ T cells (M) and percent of neutrophils (N) in colonic LP of littermate IL-1R+/-and IL-1R-/-at 3 weeks of age. (O) UMAP of type 3 immunity score and Il1r1 expression on total thymocytes from day 0 mouse (P) Percent of IL-17+ γδ T cells in 3-week-old TCRδ-/-adoptively transferred with IL-1R+/-or IL-1R-/-thymus cells. (Q) UMAP of IL-1R1 expression in total CD45+ cells in human fetal thymus γδ T cells. (R, S ) Percent of IFNγ (R) and IL-17 (S) of Vγ9+ γδ T cells in human cord blood following stimulation with IL-1β and zoledronate. (B, C, D, E, G, I-N, P, R, and S) Each dot represents cells from an individual mouse. Data are represented as mean with SD. Graphs are pooled data from at least two independent experiments with atleast n = 3 in each experiment. (H) Contour plots are representative at least two independent experiments. Numbers indicate frequency of cells within each quadrant or box. (B, C, G) Data were analyzed using Kruskal-Wallis ANOVA test or (D, E, I-N and P) Mann-Whitney non-parametric unpaired T test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p <0.0001).

Journal: bioRxiv

Article Title: Early life γδ T cell activation enforces intestinal barrier integrity during intergenerational C. difficile colonization

doi: 10.64898/2026.04.28.721483

Figure Lengend Snippet: The early life IL-17 response is dependent on IL-1 β produced by infiltrating myeloid cells. (A) Differential abundance using MiloR analysis of single cell sequencing of total CD45+ cells of weanlings and adults. (B, C) Total number of neutrophils(B) and monocytes (C) in the colon normalized to weight of the tissue of mice at indicated ages. (D, E) Total number of neutrophils (D) and monocytes (E) in the colon lamina propria of 3-week old littermate IL-17+/-and IL17-/-mice. (F) UMAP of Il1b expression in weanling and adult colon CD45+ cells. (G) Expression of Il1b in total colon of mice at indicated ages. (H, I) Representative flow plot (H) and percent (I) of pro-IL-1β+ myeloid cells in the LP of weanling and adult colons. (J) ELISA quantification of IL-1β in the supernatant of colon explants of weanling and adult mice, normalized to weight of tissue. (K, L) Percent of IL-17+ γδ T cells (K) and percent of neutrophils (L) in colonic LP of control mice and mice treated with anti-IL-1R in vivo antibody from 2-3 weeks. (M, N) Percent of IL-17+ γδ T cells (M) and percent of neutrophils (N) in colonic LP of littermate IL-1R+/-and IL-1R-/-at 3 weeks of age. (O) UMAP of type 3 immunity score and Il1r1 expression on total thymocytes from day 0 mouse (P) Percent of IL-17+ γδ T cells in 3-week-old TCRδ-/-adoptively transferred with IL-1R+/-or IL-1R-/-thymus cells. (Q) UMAP of IL-1R1 expression in total CD45+ cells in human fetal thymus γδ T cells. (R, S ) Percent of IFNγ (R) and IL-17 (S) of Vγ9+ γδ T cells in human cord blood following stimulation with IL-1β and zoledronate. (B, C, D, E, G, I-N, P, R, and S) Each dot represents cells from an individual mouse. Data are represented as mean with SD. Graphs are pooled data from at least two independent experiments with atleast n = 3 in each experiment. (H) Contour plots are representative at least two independent experiments. Numbers indicate frequency of cells within each quadrant or box. (B, C, G) Data were analyzed using Kruskal-Wallis ANOVA test or (D, E, I-N and P) Mann-Whitney non-parametric unpaired T test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p <0.0001).

Article Snippet: IL-1r1-/-mice (C57BL/6, No.003245) were obtained from Jackson laboratories.

Techniques: Produced, Single Cell, Sequencing, Expressing, Enzyme-linked Immunosorbent Assay, Control, In Vivo, MANN-WHITNEY